P-117: The In Vitro Effect of Leptin on Semen Quality of Water Buffalo (Bubal Us Bubalis) Bulls
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Abstract:
Background: Several studies have recently investigated the role of leptin, the polypeptide hormone secreted primarily by white adipose tissue, in the reproduction of rodents, humans, and domestic animals. Materials and Methods: To investigate the in vitro effect of leptin on buffalo semen quality in equilibrated and frozen thawed semen, five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37°C with tris-based extender containing 0 (control), 10, 50, 100, and 200 ng/mL leptin. After keeping diluted semen 4 hours in refrigerator to reach to the equilibration time, the semen was packed in 0.5 mL French straws and frozen in liquid nitrogen. The sperm motility, viability, and DNA fragmentation were evaluated at equilibration time and after thawing the frozen semen. Results: Results showed that in equilibrated semen adding 10 ng/ml leptin on semen extender significantly increased sperm motility and viability compared to control group, but 200 ng/ml leptin decreased semen parameter significantly. In the frozen thawed semen, all leptin concentrations decreased sperm motility and viability whit the significant decrease of 100 and 200 ng/ ml. There was no significant difference between leptin and sperm DNA fragmentation in all group in the equilibrated and frozen thawed semen. Conclusion: This study showed that although in vitro addition of 10 ng/ml leptin can increased sperm motility and viability in equilibrated semen, but all leptin concentrations decreased these parameters in buffalo bulls frozen thawed semen with the significant decrease of 100 and 200 ng/ml which may be is due to the role of leptin in sperm capacitation.
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Journal title
volume 6 issue 2
pages -
publication date 2012-09-01
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